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 | Acesso ao texto completo restrito à biblioteca da Embrapa Suínos e Aves. Para informações adicionais entre em contato com cnpsa.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Suínos e Aves. |
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Data corrente: |
13/06/2016 |
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Data da última atualização: |
13/06/2016 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ALMEIDA, F. de; MEDEIROS, M. I. C.; KICH, J. D.; FALCÃO, J. P. |
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Afiliação: |
FERNANDA DE ALMEIDA, USP/Faculdade de Ciências farmacêuticas de Ribeirão Preto; MARTA INÊS CAZENTINI MEDEIROS, Instituto Adolfo Lutz; JALUSA DEON KICH, CNPSA; JULIANA PFRIMER FALCÃO, USP/Faculdade de Ciências Farmacêuticas de Ribeirão Preto. |
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Título: |
Virulence-associated genes, antimicrobial resistance and molecular typing of salmonella typhimurium strains isolated from swine from 2000 to 2012 in Brazil. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Journal of Applied Microbiology, v. 120, n. 6, p. 1677-1690, 2016. |
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DOI: |
10.1111/jam.13110 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract Aims: The aims of this study were to assess the pathogenic potential, antimicrobial resistance and genotypic diversity of Salmonella Typhimurium strains isolated in Brazil from swine (22) and the surrounding swine environment (5) from 2000 to 2012 and compare them to the profiles of 43 human strains isolated from 1983 to 2010, which had been previously studied. Methods and Results: The presence of 12 SPI-1, SPI-2 and plasmid genes was assessed by PCR, the antimicrobial susceptibility to 13 antimicrobials was determined by the disc diffusion assay and genotyping was performed using pulsed-field gel electrophoresis (PFGE), multiple-locus variable-number of tandem repeats analysis (MLVA) and ERIC-PCR. More than 778% of the swine strains carried 10 or more of the virulence markers. Ten (37%) strains isolated from swine were multi-drug resistant (MDR). All the molecular typing techniques grouped the strains in two main clusters. Some strains isolated from swine and humans were allocated together in the PFGE-B2, MLVA-A1, MLVA-B and ERIC-A1 clusters. Conclusions: The genotyping results suggest that some strains isolated from swine and humans may descend from a common subtype and may indicate a possible risk of MDR S. Typhimurium with high frequency of virulence genes isolated from swine to contaminate humans in Brazil. Significance and Impact of the Study: This study provided new information about the pathogenic potential, antimicrobial resistance and genotypic diversity of S. Typhimurium isolates from swine origin in Brazil, the fourth largest producer of pigs worldwide. MenosAbstract Aims: The aims of this study were to assess the pathogenic potential, antimicrobial resistance and genotypic diversity of Salmonella Typhimurium strains isolated in Brazil from swine (22) and the surrounding swine environment (5) from 2000 to 2012 and compare them to the profiles of 43 human strains isolated from 1983 to 2010, which had been previously studied. Methods and Results: The presence of 12 SPI-1, SPI-2 and plasmid genes was assessed by PCR, the antimicrobial susceptibility to 13 antimicrobials was determined by the disc diffusion assay and genotyping was performed using pulsed-field gel electrophoresis (PFGE), multiple-locus variable-number of tandem repeats analysis (MLVA) and ERIC-PCR. More than 778% of the swine strains carried 10 or more of the virulence markers. Ten (37%) strains isolated from swine were multi-drug resistant (MDR). All the molecular typing techniques grouped the strains in two main clusters. Some strains isolated from swine and humans were allocated together in the PFGE-B2, MLVA-A1, MLVA-B and ERIC-A1 clusters. Conclusions: The genotyping results suggest that some strains isolated from swine and humans may descend from a common subtype and may indicate a possible risk of MDR S. Typhimurium with high frequency of virulence genes isolated from swine to contaminate humans in Brazil. Significance and Impact of the Study: This study provided new information about the pathogenic potential, antimicrobial resistance and genotypic diversity o... Mostrar Tudo |
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Thesagro: |
Bactéria; Microbiologia; Patogenicidade; Produção animal; Salmonella Typhimurium; Suíno. |
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Thesaurus Nal: |
Swine production; Virulence. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02445naa a2200265 a 4500
001 2046836
005 2016-06-13
008 2016 bl uuuu u00u1 u #d
024 7 $a10.1111/jam.13110$2DOI
100 1 $aALMEIDA, F. de
245 $aVirulence-associated genes, antimicrobial resistance and molecular typing of salmonella typhimurium strains isolated from swine from 2000 to 2012 in Brazil.$h[electronic resource]
260 $c2016
520 $aAbstract Aims: The aims of this study were to assess the pathogenic potential, antimicrobial resistance and genotypic diversity of Salmonella Typhimurium strains isolated in Brazil from swine (22) and the surrounding swine environment (5) from 2000 to 2012 and compare them to the profiles of 43 human strains isolated from 1983 to 2010, which had been previously studied. Methods and Results: The presence of 12 SPI-1, SPI-2 and plasmid genes was assessed by PCR, the antimicrobial susceptibility to 13 antimicrobials was determined by the disc diffusion assay and genotyping was performed using pulsed-field gel electrophoresis (PFGE), multiple-locus variable-number of tandem repeats analysis (MLVA) and ERIC-PCR. More than 778% of the swine strains carried 10 or more of the virulence markers. Ten (37%) strains isolated from swine were multi-drug resistant (MDR). All the molecular typing techniques grouped the strains in two main clusters. Some strains isolated from swine and humans were allocated together in the PFGE-B2, MLVA-A1, MLVA-B and ERIC-A1 clusters. Conclusions: The genotyping results suggest that some strains isolated from swine and humans may descend from a common subtype and may indicate a possible risk of MDR S. Typhimurium with high frequency of virulence genes isolated from swine to contaminate humans in Brazil. Significance and Impact of the Study: This study provided new information about the pathogenic potential, antimicrobial resistance and genotypic diversity of S. Typhimurium isolates from swine origin in Brazil, the fourth largest producer of pigs worldwide.
650 $aSwine production
650 $aVirulence
650 $aBactéria
650 $aMicrobiologia
650 $aPatogenicidade
650 $aProdução animal
650 $aSalmonella Typhimurium
650 $aSuíno
700 1 $aMEDEIROS, M. I. C.
700 1 $aKICH, J. D.
700 1 $aFALCÃO, J. P.
773 $tJournal of Applied Microbiology$gv. 120, n. 6, p. 1677-1690, 2016.
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Registro original: |
Embrapa Suínos e Aves (CNPSA) |
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Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Florestas. Para informações adicionais entre em contato com cnpf.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Florestas. |
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Data corrente: |
06/03/2009 |
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Data da última atualização: |
02/10/2012 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
ZÜGE, F. T. E.; PAULINO, B. M.; AVERO, C. C.; ZÜGE, E. T. E.; RIBASKI, J.; VARELLA, A. C. |
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Afiliação: |
FILEMON THOMAS EINHARDT ZÜGE, UERGS; BRUNO MORAES PAULINO, UERGS; CÁTIA CILENE SARAIVA AVERO, UERGS; ELDO TIMOTEO EINHARDT ZÜGE, UERGS; JORGE RIBASKI, CNPF; ALEXANDRE COSTA VARELLA, CPPSUL. |
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Título: |
Avaliação do comportamento luminoso e da produção de forragem em diferentes espaçamentos arbóreos de um sistema silvopastoril em Alegrete/RS. |
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Ano de publicação: |
2007 |
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Fonte/Imprenta: |
In: JORNADA DE PÓS-GRADUAÇÃO E PESQUISA, 5.; MOSTRA DE INICIAÇÃO CIENTÍFICA, 5.; MOSTRA DE INICIAÇÃO CIENTÍFICA JÚNIOR, 3.; MINICURSOS, 2007, Caçapava do Sul. Anais... Bagé: LEB, 2007. CONGREGA URCAMP. Resumo. |
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Páginas: |
p. 124. |
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Idioma: |
Português |
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Thesagro: |
Árvore; Espaçamento; Forragem. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 00830naa a2200217 a 4500
001 1315428
005 2012-10-02
008 2007 bl uuuu u00u1 u #d
100 1 $aZÜGE, F. T. E.
245 $aAvaliação do comportamento luminoso e da produção de forragem em diferentes espaçamentos arbóreos de um sistema silvopastoril em Alegrete/RS.
260 $c2007
300 $ap. 124.
650 $aÁrvore
650 $aEspaçamento
650 $aForragem
700 1 $aPAULINO, B. M.
700 1 $aAVERO, C. C.
700 1 $aZÜGE, E. T. E.
700 1 $aRIBASKI, J.
700 1 $aVARELLA, A. C.
773 $tIn: JORNADA DE PÓS-GRADUAÇÃO E PESQUISA, 5.; MOSTRA DE INICIAÇÃO CIENTÍFICA, 5.; MOSTRA DE INICIAÇÃO CIENTÍFICA JÚNIOR, 3.; MINICURSOS, 2007, Caçapava do Sul. Anais... Bagé: LEB, 2007. CONGREGA URCAMP. Resumo.
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